BIOFACT

PRODUCT

BioFACT™ Taq DNA Polymerase

The Most Common PCR Enzyme, Taq DNA Polymerase

  • Standard

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    Cat. No. Product Size
    ST116-500 BioFACT™ Taq DNA Polymerase with 10mM dNTP Mix (each 10mM, 0.4㎖) 500 U
    ST116-25h BioFACT™ Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 2㎖) 2,500 U (500 U x 5 ea)
    ST116-50h BioFACT™ Taq DNA Polymerase with 10mM dNTP Mix(each 10mM, 4㎖) 5,000 U (500 U x 10 ea)
    ST166-500 BioFACT™ Taq DNA Polymerase with 10mM dNTP(U) Mix (each 10mM, 0.4㎖) 500 U
    ST111-500 BioFACT™ Taq DNA Polymerase, w/o dNTP, w/o Band Helper™ 500 U

  • Master Mix

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    Cat. No. Product Size
    ST301-50h BioFACT™ 2X Taq PCR Master Mix 1, w/o Band Helper™, with dye 1 ㎖ x 5 ea
    ST302-50h BioFACT™ 2X Taq PCR Master Mix 2, with 0.5X Band Helper™, with dye 1 ㎖ x 5 ea

  • Pre-Mix

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    Cat. No. Product Size
    ST402-096 BioFACT™ 2X Taq PCR Pre-Mix, with Band Helper™, with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
    ST402-480 BioFACT™ 2X Taq PCR Pre-Mix, with Band Helper™, with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)

  • Description

    BioFACT™ Taq DNA Polymerase is a thermostable DNA polymerase from Thermus aquaticus that possesses 5' to 3' polymerase activity. It is the enzyme most widely used in various PCR-based techniques from routine PCR applications such as genotyping, RT-PCR for diagnosis and colony PCR to demanding PCR applications.

    ■  Feature
    • Source : Thermus aquaticus 
    • 5' →  3' exonuclease activity : Yes
    • 3' →  5' exonuclease activity (fidelity) : No
    • Amplification size : < 5 kb PCR
    • Hot start activity : No 
    • A-tailing : Yes
    • Error rate : 12 - 13 bp error / 106 bp

    ■  Application
    • Routine PCR
    • PCR for diagnosis
    • TA Cloning
    • Colony PCR
    • UDG system

  • QC Data

  • Etc.

    Reference
    1.  J Biotechnol. 2015 Aug 20;208:1-10. doi: 10.1016/ j.jbiotec.2015.05.006. Epub 2015 May 23. Production of 13S-hydroxy-9(Z)-octadecenoic acid from linoleic acid by  whole recombinant cells expressing linoleate 13-hydratase from Lactobacillus acidophilus. Park JY1, Lee SH1, Kim KR1, Park JB2, Oh DK3.
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