BIOFACT

PRODUCT

BioFACT™ F-Star Taq DNA Polymerase

Optimal Taq DNA Polymerase for Less Than 3kb Specific PCR

  • Standard

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    Cat. No. Product Size
    FS106-500 BioFACT™ F- Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 0.4 ㎖) 500 U
    FS106-25h BioFACT™ F- Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 2 ㎖) 2,500 U (500 U x 5 ea)
    FS106-50h BioFACT™ F- Star Taq DNA Polymerase with 10 mM dNTP Mix (each 10 mM, 4 ㎖) 5,000 U (500 U x 10 ea) 1

  • Master Mix

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    Cat. No. Product Size
    FS301-50h BioFACT™ 2X F-Star Taq PCR Master Mix 1 ㎖ x 5 ea

  • Pre-Mix

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    Cat. No. Product Size
    FS401-096 BioFACT™ 2X F-Star Taq PCR Pre-Mix, Standard, w/o Band Helper™, with dye, total 30 ㎕ reactions 96 Tubes (8 strip x 12 ea)
    FS401-480 BioFACT™ 2X F-Star Taq PCR Pre-Mix, Standard, w/o Band Helper™, with dye, total 30 ㎕ reactions 480 Tubes (8 strip x 60 ea)

  • Description

    BioFACT™ F-Star Taq DNA Polymerase is designed to be highly specific to the target gene through chemical-mediated Hot Start system. It has similar features with BioFACT™ H-Star Taq DNA Polymerase and especially it is suitable to amplify DNA fragments larger than 1kb. In case of chemical-mediated Hot Start PCR enzymes, its amplification efficiency and productivity may be lower than general Taq DNA Polymerase. In this case, it can be supplemented by increasing the cycle.

    ■  Feature
    • Source : Thermus aquaticus 
    • 5' →  3' exonuclease activity : Yes
    • 3' →  5' exonuclease activity (fidelity) : No
    • Amplification size : < 3 kb PCR 
    • A-tailing : Yes
    • Hot Start activity (Chemical-mediated Hot Start)
    • All the advantages of Hot Start PCR enzyme and general Taq DNA Polymerase
    • Error rate : 12 - 13bp error / 106 bp

    ■  Application
    • Routine PCR
    • SNP / Genotyping
    • TA Cloning
    • Suitable for UDG system

  • QC Data

  • Etc.

    Reference
    1. Bioresour Technol. 2015 Dec 5;202:125-132. doi: 10.1016/j.biortech.2015.11.085. [Epub ahead of print] Monitoring the microbial community shift throughout the shock changes of hydraulic retention time in an anaerobic moving bed membrane bioreactor. Win TT1, Kim H2, Cho K2, Song KG3, Park J4
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